Vesicular Transport Part A 9780080859293, 9780125641319, 0125641311

Table of contents :
Content:
Series Editor
Page ii

Edited by
Page iii

Copyright page
Page iv

Dedication
Page v

Contributors
Pages xv-xviii

Preface
Pages xix-xxii
Alan M. Tartakoff

Part I. Gaining Access to the Cytoplasm
Pages 1-2

Chapter 1 Lysed Chromatophores: A Model System for the Study of Bidirectional Organelle Transport Original Research Article
Pages 3-24
Leah T. Haimo, Moshe M. Rozdzial

Chapter 2 Digitonin Permeabilization Procedures for the Study of Endosome Acidification and Function Original Research Article
Pages 25-43
Ruben Diaz, Philip D. Stahl

Chapter 3 ATP Permeabilization of the Plasma Membrane Original Research Article
Pages 45-61
Thomas H. Steinberg, Samuel C. Silverstein

Chapter 4 Poration by α-Toxin and Streptolysin O: An Approach to Analyze Intracellular Processes Original Research Article
Pages 63-90
Gudrun Ahnert-Hilger, Wolfgang Mach, Karl Josef Föhr, Manfred Gratzl

Chapter 5 Preparation of Semiintact Chinese Hamster Ovary Cells for Reconstitution of Endoplasmic Reticulum-to-Golgi Transport in a Cell-Free System Original Research Article
Pages 91-102
C.J.M. Beckers, D.S. Keller, W.E. Balch

Chapter 6 Perforated Cells for Studying Intracellular Membrane Transport Original Research Article
Pages 103-126
Mark K. Bennett, Angela Wandinger-Ness, Ivan De Curtis, Claude Antony, Kai Simons, Jürgen Kartenbeck

Chapter 7 Reconstitution of Protein Transport Using Broken Yeast Spheroplasts Original Research Article
Pages 127-141
David Baker, Randy Schekman

Chapter 8 Reconstitution of Transport from the ER to the Golgi Complex in Yeast Using Microsomes and Permeabilized Yeast Cells Original Research Article
Pages 143-154
Hannele Ruohola, Alisa Kastan Kabcenell, Susan Ferro-Novick

Chapter 9 Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein Original Research Article
Pages 155-176
Harma Ellens, Stephen Doxsey, Jeffrey S. Glenn, Judith M. White

Part II. Cell-Free Reconstitution of Transport: Proteins That Interact with the Endodomain of Membranes
Pages 177-178

Chapter 10 Reconstitution of Intracellular Vesicle Fusion in a Cell-Free System after Receptor-Mediated Endocytosis Original Research Article
Pages 179-196
Luis S. Mayorga, Ruben Diaz, Philip D. Stahl

Chapter 11 Fusion of Endocytic Vesicles in a Cell-Free System Original Research Article
Pages 197-206
Philip G. Woodman, Graham Warren

Chapter 12 Purification and Biochemical Assay of Synexin and of the Homologous Calcium-Dependent Membrane-Binding Proteins, Endonexin II and Lipocortin I Original Research Article
Pages 207-227
Harvey B. Pollard, A. Lee Burns, Eduardo Rojas, D.D. Schlaepfer, Harry Haigler, Keith Brocklehurst

Chapter 13 Characterization of Coated-Vesicle Adaptors: Their Reassembly with Clathrin and with Recycling Receptors Original Research Article
Pages 229-243
Barbara M.F. Pearse

Part III. Subcellular Fractionation Procedures
Pages 245-246

Chapter 14 Lectin—Colloidal Gold-Induced Density Perturbation of Membranes: Application to Affinity Elimination of the Plasma Membrane Original Research Article
Pages 247-263
Dwijendra Gupta, Alan M. Tartakoff

Chapter 15 Immunoisolation Using Magnetic Solid Supports: Subcellular Fractionation for Cell-Free Functional Studies Original Research Article
Pages 265-292
Kathryn E. Howell, Ruth Schmid, John Ugelstad, Jean Gruenberg

Chapter 16 Flow-Cytometric Analysis of Endocytic Compartments Original Research Article
Pages 293-317
Russell B. Wilson, Robert F. Murphy

Chapter 17 Endosome and Lysosome Purification by Free-Flow Electrophoresis Original Research Article
Pages 319-334
Mark Marsh

Chapter 18 Fractionation of Yeast Organelles Original Research Article
Pages 335-354
Nancy C. Walworth, Bruno Goud, Hannele Ruohola, Peter J. Novick

Part IV. Morphological Procedures
Pages 355-356

Chapter 19 Fluorescence Microscopy Methods for Yeast Original Research Article
Pages 357-435
John R. Pringle, Robert A. Preston, Alison E.M. Adams, Tim Stearns, David G. Drubin, Brian K. Haarer, Elizabeth W. Jones

Chapter 20 Preservation of Biological Specimens for Observation in a Confocal Fluorescence Microscope and Operational Principles of Confocal Fluorescence Microscopy Original Research Article
Pages 437-452
Robert Bacallao, Ernst H.K. Stelzer

Chapter 21 Organic-Anion Transport Inhibitors to Facilitate Measurement of Cytosolic Free Ca2+ with Fura-2 Original Research Article
Pages 453-462
Francesco Di Virgilio, Thomas H. Steinberg, Samuel C. Silverstein

Chapter 22 Postembedding Detection of Acidic Compartments Original Research Article
Pages 463-472
Richard G.W. Anderson

Chapter 23 Transmission Electron Microscopy and Immunocytochemical Studies of Yeast: Analysis of HMG—CoA Reductase Overproduction by Electron Microscopy Original Research Article
Pages 473-512
Robin Wright, Jasper Rine

Chapter 24 Postembedding Labeling on Lowicryl K4M Tissue Sections: Detection and Modification of Cellular Components Original Research Article
Pages 513-551
J. Roth

Chapter 25 Immunoperoxidase Methods for the Localization of Antigens in Cultured Cells and Tissue Sections by Electron Microscopy Original Research Article
Pages 553-569
William J. Brown, Marilyn G. Farquhar

Index
Pages 571-597