Stem Cells and Aging. Methods and Protocols [2 ed.] 978-1-4939-9712-1, 978-1-4939-9713-8

Table of contents :
Front Matter ....Pages i-xii
Assessing Muscle Stem Cell Clonal Complexity During Aging (Matthew T. Tierney, Michael J. Stec, Alessandra Sacco)....Pages 1-11
Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies (Eusebio Perdiguero, Victoria Moiseeva, Pura Muñoz-Cánoves)....Pages 13-23
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle (Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler et al.)....Pages 25-36
Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue (Meenakshi Gaur, Marek Dobke, Victoria V. Lunyak)....Pages 37-92
Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures (Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, Francis Rodier)....Pages 93-105
Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells (Courtney R. Ogando, Gilda A. Barabino, Yueh-Hsun Kevin Yang)....Pages 107-117
Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis (Klemen Čamernik, Janja Marc, Janja Zupan)....Pages 119-129
Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells (Klemen Čamernik, Janja Zupan)....Pages 131-144
Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis (Janja Zupan)....Pages 145-154
3D-Embedded Cell Cultures to Study Tendon Biology (Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer et al.)....Pages 155-165
Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis (Brooke Snetsinger, Christina K. Ferrone, Michael J. Rauh)....Pages 167-180
Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants (Sherin Saheera, Renuka R. Nair)....Pages 181-186
Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice (Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, Noël Ghanem)....Pages 187-199
Infrared Spectroscopy and Imaging in Stem Cells and Aging Research (Ceren Aksoy, Feride Severcan)....Pages 201-215
Use of U-STELA for Accurate Measurement of Extremely Short Telomeres (Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis)....Pages 217-224
Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry (Klemen Čamernik, Janja Zupan)....Pages 225-233
Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry (Rasmus Jentoft Boutrup)....Pages 235-244
Methods for Detection of Autophagy in Mammalian Cells (Bindu Singh, Sangeeta Bhaskar)....Pages 245-258
Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells (Yohei Hayashi, Yasuhisa Matsui)....Pages 259-269
Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer (Dan-Ya Wu, Xia Zhang, Yi-Liang Miao)....Pages 271-281
Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD) (Harshini Surendran, Reena J. Rathod, Rajarshi Pal)....Pages 283-298
Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury (Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko et al.)....Pages 299-310
3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol (Yuhui Lin)....Pages 311-321
Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments (Shuh-Wen Aung, Noor Hayaty Abu Kasim, Thamil Selvee Ramasamy)....Pages 323-335
CRISPR Base Editing in Induced Pluripotent Stem Cells (Ya-Ju Chang, Christine L. Xu, Xuan Cui, Alexander G. Bassuk, Vinit B. Mahajan, Yi-Ting Tsai et al.)....Pages 337-346
Back Matter ....Pages 347-354