Handbook of Single-Molecule Biophysics [1 ed.] 0387764968, 9780387764962
The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study
298 24 265KB
English Pages 626 [634] Year 2009
Table of contents :
Front Matter....Pages i-xxi
Single-Molecule Fluorescent Particle Tracking....Pages 1-18
Single-Molecule Analysis of Biomembranes....Pages 19-42
Single-Molecule Imaging in Live Cells....Pages 43-93
Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy....Pages 95-127
Single-Molecule FRET: Methods and Biological Applications....Pages 129-163
Single-Molecule Enzymology....Pages 165-182
Single-Molecule Studies of Rotary Molecular Motors....Pages 183-216
Fluorescence Correlation Spectroscopy in Living Cells....Pages 217-241
Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy....Pages 243-263
Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores....Pages 265-291
Nanopores: Generation, Engineering, and Single-Molecule Applications....Pages 293-339
Single-Molecule Manipulation Using Optical Traps....Pages 341-370
Magnetic Tweezers for Single-Molecule Experiments....Pages 371-395
Folding of Proteins under Mechanical Force....Pages 397-406
Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy....Pages 407-447
Probing Single Membrane Proteins by Atomic Force Microscopy....Pages 449-485
High-Speed Atomic Force Microscopy....Pages 487-523
Recognition Imaging Using Atomic Force Microscopy....Pages 525-554
Atomic Force Microscopy of Protein–Protein Interactions....Pages 555-570
A New Approach to Analysis of Single-Molecule Force Measurements....Pages 571-589
Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation....Pages 591-610
Back Matter....Pages 611-626
Front Matter....Pages i-xxi
Single-Molecule Fluorescent Particle Tracking....Pages 1-18
Single-Molecule Analysis of Biomembranes....Pages 19-42
Single-Molecule Imaging in Live Cells....Pages 43-93
Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy....Pages 95-127
Single-Molecule FRET: Methods and Biological Applications....Pages 129-163
Single-Molecule Enzymology....Pages 165-182
Single-Molecule Studies of Rotary Molecular Motors....Pages 183-216
Fluorescence Correlation Spectroscopy in Living Cells....Pages 217-241
Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy....Pages 243-263
Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores....Pages 265-291
Nanopores: Generation, Engineering, and Single-Molecule Applications....Pages 293-339
Single-Molecule Manipulation Using Optical Traps....Pages 341-370
Magnetic Tweezers for Single-Molecule Experiments....Pages 371-395
Folding of Proteins under Mechanical Force....Pages 397-406
Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy....Pages 407-447
Probing Single Membrane Proteins by Atomic Force Microscopy....Pages 449-485
High-Speed Atomic Force Microscopy....Pages 487-523
Recognition Imaging Using Atomic Force Microscopy....Pages 525-554
Atomic Force Microscopy of Protein–Protein Interactions....Pages 555-570
A New Approach to Analysis of Single-Molecule Force Measurements....Pages 571-589
Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation....Pages 591-610
Back Matter....Pages 611-626
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- Antoine Oijen (eds.)
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