Handbook of Isoelectric Focusing and Proteomics [1 ed.] 0120887525, 9780120887521, 9780080533629

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool

178 64 243KB

English Pages 1-334 [357] Year 2005

Table of contents :
Content:
Preface
Pages xi-xiii
David Garfin, Satinder Ahuja

Contributors
Pages xv-xvi

Incidents of travel in IEF and IPGS
Pages xvii-xxii
Pier Giorgio Righetti

I Overview Original Research Article
Pages 1-12
David E. Garfin, Satinder Ahuja

2 Evolution and development of isoelectric focusing Original Research Article
Pages 13-39
Ákos Végvári, Ferenc Kilár

3 Theory and simulation of isoelectric focusing Original Research Article
Pages 41-68
T.L. Sounart, P.A. Safier, J.C. Baygents

4 Generation of pH gradients Original Research Article
Pages 69-92
Tom Berkelman

5 Slab gel IEF Original Research Article
Pages 93-122
Reiner Westermeier

6 Two-dimensional gel electrophoresis Original Research Article
Pages 123-145
Mark P. Molloy, Michael T. McDowell

7 Some practices and pitfalls of sample preparation for isoelectric focusing in proteomics Original Research Article
Pages 147-164
Ben Herbert

8 Protein detection and imaging in IEF gels Original Research Article
Pages 165-179
Wayne F. Patton

9 Capillary isoelectric focusing Original Research Article
Pages 181-210
Tim Wehr

10 Free-flow isoelectric focusing Original Research Article
Pages 211-245
Peter J.A. Weber, Gerhard Weber, Christoph Eckerskorn, Ulrich Schneider, Anton Posch

11 Isoelectric focusing and proteomics Original Research Article
Pages 247-264
Melanie Y. White, Stuart J. Cordwell

12 Chromatofocusing Original Research Article
Pages 265-296
David Anderson

13 Alternative electrofocusing methods Original Research Article
Pages 297-319
Cornelius F. Ivory

Index
Pages 321-334

Handbook of Isoelectric Focusing and Proteomics [1 ed.]
0120887525, 9780120887521, 9780080533629

Author / Uploaded
David Garfin and Satinder Ahuja (Eds.)

Similar Topics
Biology
Biochemistry

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