Confocal Microscopy [1 ed.] 9780121822088, 0121822087

Table of contents :
Cover......Page 1
Preface......Page 2
Contributors......Page 3
01 Theoretical Basis of Confocal Microscopy......Page 7
02 Prractical Considerations in Acquiring Biological Signals from Confocal Microscope......Page 24
03 Equipment for Mass Storage and Processing of Data......Page 32
04 Antifading Agents for Confocal Fluorescence Microscopy......Page 58
05 Mounting Techniques for Confocal Microscopy......Page 83
06 Preparation of Whole Mounts and Thick Sections for Confocal Microscopy......Page 90
07 Cytotomography......Page 114
08 Use of Confocal Microscopy to Investigate Cell Structure and Function......Page 125
09 Combining Laser Scanning Confocal Microscopy and Electron Microscopy......Page 141
10 Construction of Line-Scan Confocal Microscope for Physiological Recording......Page 159
11 Three-Dimensional Visualization of Cytoskeleton by Confocal Laser Scanning Microscopy......Page 178
12 Chromosome Spread for Confocal Microscopy......Page 195
13 Visualization of Nuclear Pore Complex and Its Distribution b y Confocal Laser Scanning Microscopy......Page 212
14 Measurement of Tissue Thickness Using Confocal Microscopy......Page 236
15 Measurements of Vascular Remodeling by Confocal Microscopy......Page 252
16 Cell Volume Measurements by Fluorescence Confocal Microscopy: Theoretical and Practical Aspects......Page 280
17 Volume Measurements in Confocal Microscopy......Page 302
18 Quantitation of Phagocytosis by Confocal Microscopy......Page 322
19 Measurement of Secretion in Confocal Microscopy......Page 331
20 Receptor-Ligand Internalization......Page 343
21 Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy......Page 354
22 Localization of Proteases and Peptide Receptors by Confocal Microscopy......Page 371
23 Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy......Page 397
24 Confocal Imaging Analysis of Intracellular Ions in Mixed Cellular Systems or in Situ Using Two Types of Confocal Microscopic Systems......Page 426
25 Confocal Ca 2÷ Imaging of Organelles, Cells,Tissues, and Organs......Page 442
26 Using Confocal Microscopy and the Fluorescent Indicator, 6-Methoxy-N-ethylquinolinium Iodide, to Measure Changes in Introcellular Chloride......Page 470
27 Measurement of Mineral Gain and Loss on Dental Enamel and Dentin Using Confocal Microscopy......Page 483
28 Characterization of Ocular Cellular and Extracellular Structures Using Confocal Microscopy andComputerized Three-Dimensional Reconstruction......Page 494
29 Multiphoton Excitation Microscopy,Confocal Microscopy, and Spectroscopy of Living Cells and Tissues; Functional Metabolic Imaging of Human Skin in Vivo......Page 512
30 Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye......Page 535
31 In Vivo Imaging of Mammalian Central Nervous System Neurons w i t h t h e in Vivo Confoeal Neuroimaging (ICON) Method......Page 562
32 Identification of Viral Infection by Confocal Microscopy......Page 570
33 Using Confocal Microscopy to Study Virus Binding and Entry into Cells......Page 588
34 Fluorescent Labels , Confocal Microscopy, and Quantitative Image Analysis in Study of Fungal Biology......Page 604
Author Index......Page 621
Subject Index......Page 643