X-Chromosome Inactivation: Methods and Protocols 978-1-4939-8765-8, 978-1-4939-8766-5

Table of contents :
Front Matter ....Pages i-xi
Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs (Asun Monfort, Giulio Di Minin, Anton Wutz)....Pages 1-18
Unbiased Genetic Screen to Identify Factors Involved in X-Chromosome Inactivation Using a Pooled Bar-Coded shRNA Library (Benoit Moindrot, Neil Brockdorff)....Pages 19-36
ChIRP-MS: RNA-Directed Proteomic Discovery (Ci Chu, Howard Y. Chang)....Pages 37-45
Manipulation of Xist Imprinting in Mouse Preimplantation Embryos (Atsushi Fukuda, Akihiro Umezawa, Hidenori Akutsu)....Pages 47-53
Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status (Kimiko Inoue, Shogo Matoba, Atsuo Ogura)....Pages 55-65
Live Imaging of Xist RNA (Osamu Masui, Edith Heard, Haruhiko Koseki)....Pages 67-72
Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics (Shin Kobayashi)....Pages 73-89
Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes (Yuko Sato, Timothy J. Stasevich, Hiroshi Kimura)....Pages 91-102
Visualization of Hidden Epitopes at the Inactive X Chromosome (Emma R. Stewart, Dawn Coverley)....Pages 103-112
Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation (Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa)....Pages 113-129
Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos (Aristea Magaraki, Agnese Loda, Joost Gribnau, Willy M. Baarends)....Pages 131-147
Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos (Ikuhiro Okamoto)....Pages 149-159
RNA-FISH and Immunofluorescence of Mouse Preimplantation and Postimplantation Embryos (Hirosuke Shiura, Yuka Sakata, Kuniya Abe, Takashi Sado)....Pages 161-176
Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation (Marissa Cloutier, Clair Harris, Srimonta Gayen, Emily Maclary, Sundeep Kalantry)....Pages 177-203
X-Chromosome Inactivation and Escape from X Inactivation in Mouse (Wenxiu Ma, Giancarlo Bonora, Joel B. Berletch, Xinxian Deng, William S. Noble, Christine M. Disteche)....Pages 205-219
Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles (Hisashi Miura, Rawin Poonperm, Saori Takahashi, Ichiro Hiratani)....Pages 221-245
Back Matter ....Pages 247-251