Ultrasensitive and Rapid Enzyme Immunoassay [1 ed.] 9780080858968, 9780444502018, 0444502017
Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well kno
139 83 226KB
English Pages ii-v, 1-334 [351] Year 1999
Table of contents :
Content:
Edited by
Page ii
Copyright page
Page iv
Preface
Page v
Eiji Ishikawa
Chapter 1 Introduction: classification of immunoassays
Pages 1-3
Chapter 2 History of ultrasensitive enzyme immunoassay
Pages 5-6
Chapter 3 Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays
Pages 7-16
Chapter 4 Methods to minimize effects of factors limiting the sensitivity
Pages 17-77
Chapter 5 Principle of the immune complex transfer enzyme immunoassay
Pages 79-95
Chapter 6 Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations
Pages 97-140
Chapter 7 Ultrasensitive and rapid enzyme immunoassay
Pages 141-176
Chapter 8 Protocol of immune complex transfer enzyme immunoassay
Pages 177-191
Chapter 9 Ultrasensitive immunoassay for haptens
Pages 193-210
Chapter 10 Properties of the reagents for enzyme-labeling
Pages 211-221
Chapter 11 Enzyme-labeling of antibodies
Pages 223-248
Chapter 12 Preparation and affinity-purification of IgG fragments
Pages 249-259
Chapter 13 Protocol for enzyme-labeling of antibodies
Pages 261-275
Chapter 14 Protocol for labeling avidin with 2,4-dinitrophenyl groups and β-D-galactosidase
Pages 277-282
Chapter 15 Enzyme-labeling of antigens
Pages 283-287
Chapter 16 Protocol for enzyme-labeling of antigens
Pages 289-302
Chapter 17 Assays of enzymes
Pages 303-309
References
Pages 311-325
Subject Index
Pages 327-334
Content:
Edited by
Page ii
Copyright page
Page iv
Preface
Page v
Eiji Ishikawa
Chapter 1 Introduction: classification of immunoassays
Pages 1-3
Chapter 2 History of ultrasensitive enzyme immunoassay
Pages 5-6
Chapter 3 Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays
Pages 7-16
Chapter 4 Methods to minimize effects of factors limiting the sensitivity
Pages 17-77
Chapter 5 Principle of the immune complex transfer enzyme immunoassay
Pages 79-95
Chapter 6 Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations
Pages 97-140
Chapter 7 Ultrasensitive and rapid enzyme immunoassay
Pages 141-176
Chapter 8 Protocol of immune complex transfer enzyme immunoassay
Pages 177-191
Chapter 9 Ultrasensitive immunoassay for haptens
Pages 193-210
Chapter 10 Properties of the reagents for enzyme-labeling
Pages 211-221
Chapter 11 Enzyme-labeling of antibodies
Pages 223-248
Chapter 12 Preparation and affinity-purification of IgG fragments
Pages 249-259
Chapter 13 Protocol for enzyme-labeling of antibodies
Pages 261-275
Chapter 14 Protocol for labeling avidin with 2,4-dinitrophenyl groups and β-D-galactosidase
Pages 277-282
Chapter 15 Enzyme-labeling of antigens
Pages 283-287
Chapter 16 Protocol for enzyme-labeling of antigens
Pages 289-302
Chapter 17 Assays of enzymes
Pages 303-309
References
Pages 311-325
Subject Index
Pages 327-334
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