Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System [1 ed.] 978-4-431-55226-0, 978-4-431-55227-7
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing
218 68 3KB
English Pages 205 Year 2015
Table of contents :
Front Matter....Pages i-viii
Front Matter....Pages 1-1
Genome Editing Using Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs)....Pages 3-24
CRISPR/Cas9: The Leading Edge of Genome Editing Technology....Pages 25-41
Front Matter....Pages 43-43
Editing Cultured Human Cells: From Cell Lines to iPS Cells....Pages 45-69
Genome Editing in Nematode....Pages 71-80
Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori , Using Genome Editing Tools....Pages 81-96
Genome Editing in Sea Urchin....Pages 97-106
Genome Editing in Ascidians....Pages 107-117
Genome Editing in Zebrafish and Medaka....Pages 119-131
Genome Editing Using Site-Specific Nucleases in Amphibians....Pages 133-149
Genome Editing in Mice Using CRISPR/Cas....Pages 151-166
Genome Editing in Mice Using TALENs....Pages 167-182
Engineered Nucleases Lead to Genome Editing Revolution in Rats....Pages 183-195
Genome Editing in Higher Plants....Pages 197-205
Front Matter....Pages i-viii
Front Matter....Pages 1-1
Genome Editing Using Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs)....Pages 3-24
CRISPR/Cas9: The Leading Edge of Genome Editing Technology....Pages 25-41
Front Matter....Pages 43-43
Editing Cultured Human Cells: From Cell Lines to iPS Cells....Pages 45-69
Genome Editing in Nematode....Pages 71-80
Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori , Using Genome Editing Tools....Pages 81-96
Genome Editing in Sea Urchin....Pages 97-106
Genome Editing in Ascidians....Pages 107-117
Genome Editing in Zebrafish and Medaka....Pages 119-131
Genome Editing Using Site-Specific Nucleases in Amphibians....Pages 133-149
Genome Editing in Mice Using CRISPR/Cas....Pages 151-166
Genome Editing in Mice Using TALENs....Pages 167-182
Engineered Nucleases Lead to Genome Editing Revolution in Rats....Pages 183-195
Genome Editing in Higher Plants....Pages 197-205
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