Sequencing of Proteins and Peptides [1 ed.] 0444802754, 9780444802750
Although there is a continuing high level of activity in direct studies on protein primary structures, the requirement f
193 64 202KB
English Pages ii-x, 1-327 [347] Year 1981
Table of contents :
Content:
Advisory board
Page ii
Edited by
Page iii
Copyright page
Page iv
Preface
Pages v-vii
List of abbreviations
Pages ix-x
Chapter 1 Introduction
Pages 1-15
Chapter 2 Preliminary characterization of the protein
Pages 17-42
Chapter 3 Specific cleavage of the protein
Pages 43-71
Chapter 4 Separation and purification of peptides
Pages 73-133
Chapter 5 Methods for the detection of peptides
Pages 135-160
Chapter 6 Methods for the detection of peptides
Pages 161-236
Chapter 7 Structures introduced by post-translational modification
Pages 237-256
Chapter 8 Deduction of the total primary structure of a protein from the sequences of constituent peptides
Pages 257-262
Chapter 9 Integral membrane proteins
Pages 263-272
Chapter 10 Applications of partial sequence analysis
Pages 273-282
Appendix I Manufacturers and suppliers of equipment and reagents for. protein sequence analysis
Pages 283-291
Appendix II Purification of solvents and reagents
Pages 291-294
Appendix III Safety in the protein chemistry laboratory
Pages 294-295
Appendix IV Notes on work with very small amounts of proteins and peptide
Pages 295-297
References
Pages 299-318
Subject index
Pages 319-327
Content:
Advisory board
Page ii
Edited by
Page iii
Copyright page
Page iv
Preface
Pages v-vii
List of abbreviations
Pages ix-x
Chapter 1 Introduction
Pages 1-15
Chapter 2 Preliminary characterization of the protein
Pages 17-42
Chapter 3 Specific cleavage of the protein
Pages 43-71
Chapter 4 Separation and purification of peptides
Pages 73-133
Chapter 5 Methods for the detection of peptides
Pages 135-160
Chapter 6 Methods for the detection of peptides
Pages 161-236
Chapter 7 Structures introduced by post-translational modification
Pages 237-256
Chapter 8 Deduction of the total primary structure of a protein from the sequences of constituent peptides
Pages 257-262
Chapter 9 Integral membrane proteins
Pages 263-272
Chapter 10 Applications of partial sequence analysis
Pages 273-282
Appendix I Manufacturers and suppliers of equipment and reagents for. protein sequence analysis
Pages 283-291
Appendix II Purification of solvents and reagents
Pages 291-294
Appendix III Safety in the protein chemistry laboratory
Pages 294-295
Appendix IV Notes on work with very small amounts of proteins and peptide
Pages 295-297
References
Pages 299-318
Subject index
Pages 319-327
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