Protein Sequencing and Identification Using Tandem Mass Spectrometry [1 ed.] 0471322490, 9780471322498
How to design, execute, and interpret experiments for protein sequencing using mass spectrometryThe rapid expansion of s
255 107 316KB
English Pages 316 Year 2000
Table of contents :
Cover......Page 1
Halftitle......Page 2
Inside Cover......Page 4
Copyright......Page 5
Dedication......Page 6
Contents......Page 7
Series Preface......Page 13
Preface......Page 14
1. AN INTRODUCTION TO PROTEIN SEQUENCING USING TANDEM MASS SPECTROMETRY......Page 17
2. THE PRIMARY STRUCTURE OF PROTEINS AND A HISTORlCAL OVERVIEW OF PROTEIN SEQUENCING......Page 22
3. FUNDAMENTAL MASS SPECTROMETRY......Page 45
4. COLLISIONALLY INDUCED DISSOCIATION OF PROTONATED PEPTIDE IONS AND THE INTERPRETATION OF PRODUCT ION SPECTRA......Page 80
5. BASIC POLYACRYLAMIDE GEL ELECTROPHORESIS......Page 133
6. THE PREPARATION OF PROTEIN DIGESTS FOR MASS SPECTROMETRIC SEQUENCING EXPERIMENTS......Page 163
7. MASS SPECTROMETRIC ANALYSIS OF TRYPTIC DIGESTS......Page 182
8. PROTEIN IDENTIFICATION BY DATABASE SEARCHING......Page 223
9. SEQUENCE ANALYSIS OF NOVEL PROTEINS......Page 254
10. THE CHARACTERIZATION OF POST-TRANSLATIONALLY MODIFIED PROTEINS USING TANDEM MASS SPECTROMETRY......Page 285
INDEX......Page 310
Cover......Page 1
Halftitle......Page 2
Inside Cover......Page 4
Copyright......Page 5
Dedication......Page 6
Contents......Page 7
Series Preface......Page 13
Preface......Page 14
1. AN INTRODUCTION TO PROTEIN SEQUENCING USING TANDEM MASS SPECTROMETRY......Page 17
2. THE PRIMARY STRUCTURE OF PROTEINS AND A HISTORlCAL OVERVIEW OF PROTEIN SEQUENCING......Page 22
3. FUNDAMENTAL MASS SPECTROMETRY......Page 45
4. COLLISIONALLY INDUCED DISSOCIATION OF PROTONATED PEPTIDE IONS AND THE INTERPRETATION OF PRODUCT ION SPECTRA......Page 80
5. BASIC POLYACRYLAMIDE GEL ELECTROPHORESIS......Page 133
6. THE PREPARATION OF PROTEIN DIGESTS FOR MASS SPECTROMETRIC SEQUENCING EXPERIMENTS......Page 163
7. MASS SPECTROMETRIC ANALYSIS OF TRYPTIC DIGESTS......Page 182
8. PROTEIN IDENTIFICATION BY DATABASE SEARCHING......Page 223
9. SEQUENCE ANALYSIS OF NOVEL PROTEINS......Page 254
10. THE CHARACTERIZATION OF POST-TRANSLATIONALLY MODIFIED PROTEINS USING TANDEM MASS SPECTROMETRY......Page 285
INDEX......Page 310
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