Monoclonal Antibody and Immunosensor Technology: The Production and Application of Rodent and Human Monoclonal Antibodies [1 ed.] 0444814124, 9780444814128
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provide
304 94 309KB
English Pages iii-xi, 1-427 [455] Year 1991
Table of contents :
Content:
Edited By
Page iii
Copyright page
Page iv
Preface
Pages v-vii
Ailsa Campbell
Acknowledgements
Page ix
Abbreviations
Page xi
Chapter 1 General properties and applications of monoclonal antibodies
Pages 1-49
Chapter 2 Assay techniques
Pages 51-115
Chapter 3 Selection of animals, tissues and cell lines
Pages 117-138
Chapter 4 Immunisation
Pages 139-162
Chapter 5 Cell culture requirements for hybridomas
Pages 163-188
Chapter 6 Fusion procedures
Pages 189-204
Chapter 7 Alternative strategies for the immortalisation of antibody producing cells
Pages 205-224
Chapter 8 Selection and cloning
Pages 225-237
Chapter 9 The use of recombinant DNA methods
Pages 239-275
Chapter 10 Antibody production and purification
Pages 277-301
Chapter 11 Characterisation of monoclonal antibodies
Pages 303-342
Chapter 12 Immunosensors
Pages 343-372
Appendix 1 - Animal handling techniques
Pages 373-387
Appendix 2
Pages 389-392
Appendix 3 - Protocols for polyacrylamide gel electrophoresis
Pages 393-400
References
Pages 401-412
Subject index
Pages 413-427
Content:
Edited By
Page iii
Copyright page
Page iv
Preface
Pages v-vii
Ailsa Campbell
Acknowledgements
Page ix
Abbreviations
Page xi
Chapter 1 General properties and applications of monoclonal antibodies
Pages 1-49
Chapter 2 Assay techniques
Pages 51-115
Chapter 3 Selection of animals, tissues and cell lines
Pages 117-138
Chapter 4 Immunisation
Pages 139-162
Chapter 5 Cell culture requirements for hybridomas
Pages 163-188
Chapter 6 Fusion procedures
Pages 189-204
Chapter 7 Alternative strategies for the immortalisation of antibody producing cells
Pages 205-224
Chapter 8 Selection and cloning
Pages 225-237
Chapter 9 The use of recombinant DNA methods
Pages 239-275
Chapter 10 Antibody production and purification
Pages 277-301
Chapter 11 Characterisation of monoclonal antibodies
Pages 303-342
Chapter 12 Immunosensors
Pages 343-372
Appendix 1 - Animal handling techniques
Pages 373-387
Appendix 2
Pages 389-392
Appendix 3 - Protocols for polyacrylamide gel electrophoresis
Pages 393-400
References
Pages 401-412
Subject index
Pages 413-427
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