Monoclonal Antibody and Immunosensor Technology: The Production and Application of Rodent and Human Monoclonal Antibodies [1 ed.] 0444814124, 9780444814128
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provide
307 94 309KB
English Pages iii-xi, 1-427 [455] Year 1991
Table of contents :
Content:
Edited By
Page iii
Copyright page
Page iv
Preface
Pages v-vii
Ailsa Campbell
Acknowledgements
Page ix
Abbreviations
Page xi
Chapter 1 General properties and applications of monoclonal antibodies
Pages 1-49
Chapter 2 Assay techniques
Pages 51-115
Chapter 3 Selection of animals, tissues and cell lines
Pages 117-138
Chapter 4 Immunisation
Pages 139-162
Chapter 5 Cell culture requirements for hybridomas
Pages 163-188
Chapter 6 Fusion procedures
Pages 189-204
Chapter 7 Alternative strategies for the immortalisation of antibody producing cells
Pages 205-224
Chapter 8 Selection and cloning
Pages 225-237
Chapter 9 The use of recombinant DNA methods
Pages 239-275
Chapter 10 Antibody production and purification
Pages 277-301
Chapter 11 Characterisation of monoclonal antibodies
Pages 303-342
Chapter 12 Immunosensors
Pages 343-372
Appendix 1 - Animal handling techniques
Pages 373-387
Appendix 2
Pages 389-392
Appendix 3 - Protocols for polyacrylamide gel electrophoresis
Pages 393-400
References
Pages 401-412
Subject index
Pages 413-427
Content:
Edited By
Page iii
Copyright page
Page iv
Preface
Pages v-vii
Ailsa Campbell
Acknowledgements
Page ix
Abbreviations
Page xi
Chapter 1 General properties and applications of monoclonal antibodies
Pages 1-49
Chapter 2 Assay techniques
Pages 51-115
Chapter 3 Selection of animals, tissues and cell lines
Pages 117-138
Chapter 4 Immunisation
Pages 139-162
Chapter 5 Cell culture requirements for hybridomas
Pages 163-188
Chapter 6 Fusion procedures
Pages 189-204
Chapter 7 Alternative strategies for the immortalisation of antibody producing cells
Pages 205-224
Chapter 8 Selection and cloning
Pages 225-237
Chapter 9 The use of recombinant DNA methods
Pages 239-275
Chapter 10 Antibody production and purification
Pages 277-301
Chapter 11 Characterisation of monoclonal antibodies
Pages 303-342
Chapter 12 Immunosensors
Pages 343-372
Appendix 1 - Animal handling techniques
Pages 373-387
Appendix 2
Pages 389-392
Appendix 3 - Protocols for polyacrylamide gel electrophoresis
Pages 393-400
References
Pages 401-412
Subject index
Pages 413-427
![Monoclonal Antibody and Immunosensor Technology: The Production and Application of Rodent and Human Monoclonal Antibodies [1 ed.]
0444814124, 9780444814128](https://ebin.pub/img/200x200/monoclonal-antibody-and-immunosensor-technology-the-production-and-application-of-rodent-and-human-monoclonal-antibodies-1nbsped-0444814124-9780444814128.jpg)
![Monoclonal antibodies: principles and practice : production and application of monoclonal antibodies in cell biology, biochemistry and immunology [3 ed.]
0122870239, 9780122870231](https://ebin.pub/img/200x200/monoclonal-antibodies-principles-and-practice-production-and-application-of-monoclonal-antibodies-in-cell-biology-biochemistry-and-immunology-3nbsped-0122870239-9780122870231.jpg)
![Current Trends in Monoclonal Antibody Development and Manufacturing [1 ed.]
0387766421, 9780387766423, 9780387766430](https://ebin.pub/img/200x200/current-trends-in-monoclonal-antibody-development-and-manufacturing-1nbsped-0387766421-9780387766423-9780387766430.jpg)
![Monoclonal Antibodies in Biotechnology: Theoretical and Practical Aspects [1St Edition]
0521258901, 9780511565137, 9780521258906](https://ebin.pub/img/200x200/monoclonal-antibodies-in-biotechnology-theoretical-and-practical-aspects-1st-edition-0521258901-9780511565137-9780521258906-r-3669834.jpg)
![Monoclonal Antibodies in Biotechnology: Theoretical and Practical Aspects [1St Edition]
0521258901, 9780511565137, 9780521258906](https://ebin.pub/img/200x200/monoclonal-antibodies-in-biotechnology-theoretical-and-practical-aspects-1st-edition-0521258901-9780511565137-9780521258906.jpg)