Table of contents : Table of Contents......Page 6 Contributors to Volume 151......Page 10 Menashe Marcus......Page 14 Preface......Page 16 VOLUMES IN SERIES......Page 18 Section I Cell Lines Useful for Genetic Analysis......Page 32 Chinese Hamster Ovary Cells......Page 34 Cultured 549 Mouse T Lymphoma Cells......Page 40 Liver Cell Lines......Page 50 HeLa Cell Lines......Page 69 Somatic Cell Genetic Analysis of Myelomas and Hybridomas......Page 81 Mouse Teratocarcinoma Cells......Page 97 Section II Special Techniques for Mutant Selection......Page 114 Amplification of Genes in Somatic Mammalian Cells......Page 116 Replica Plating and Delection of Antigen Variants......Page 135 Drug-Resistant Mutants: Selection and and Dominance Analysis......Page 144 Suicide Selection of Mammalian Cell Mutants......Page 152 Use of Tritium-Labeled Precursors to Select Mutants......Page 162 Construction of Immunotoxins Using Pseudomonas Exotoxin A......Page 170 Isolation of Temperature-Sensitive Mutants......Page 176 Fluorescence-Activated Cell Sorting of Hybrid and Transfected Cells......Page 181 Section III Genetic Mapping and Analysis......Page 198 Isozyme Identification of Chromosomes inInterspecific Somatic Cell Hybrids......Page 200 Principles of Electrofusion and Electropermeabilizalion......Page 225 Cell Enucleation. Cybrids. Reconstituted Cells. and Nuclear Hybrids......Page 256 Toxin-Antiloxin Selection for Isolating Helerobryonsand Cell Hybrids......Page 272 Chromosome Sorting by Flow Cytometry......Page 287 Methods for Chromosome Banding of Human and Experimental Tumors in Vitro......Page 302 In Silll Hybridization of Metaphase and Prometaphase Chromosomes......Page 314 Gene Mapping with Sorted Chromosomes......Page 327 Transfer and Selective Retention of Single Specific Human Chromosomes via Microcell·Mediated Chromosome Transfer......Page 348 Section IV Isolation and Detection of Mutant Genes......Page 362 Strategies for Isolation of Mutant Genes......Page 364 Use of in-Gel DNA Renaturation for Detection and Cloning of Amplified Genes......Page 367 Detection and Characterization or Specific mRNA by Microinjection and Complementation of Mutant Cells......Page 406 Use or the CAT Reporter Gene ror Optimization of Gene Transfer into Eukaryotic Cells......Page 417 Cosmid Vector Systems for Genomic DNA Cloning......Page 432 Preparation of a Genomic Cosmid Library......Page 440 Colony Screening orGcnomic Cosmid Libraries......Page 451 Rescue of Genes Transferred from Cosmid Libraries......Page 461 Phage-Mediated Transduction of cDNA Expression Libraries into Mammalian Cells......Page 469 Sib Selection......Page 480 Repetitive Ooning or Mutant Genes Using Locus-Specific Sticky Ends......Page 484 Strategies ror Mapping and Cloning Macroregions of Mammalian Genomes......Page 496 Insertional Mutagenesis to Isolate Genes......Page 524 Microcloning of Mammalian Metaphase Chromosomes......Page 538 Section V Gene Regulation in Tissue Culture......Page 552 Antisense RNA Inhibition or Endogenous Genes......Page 554 In Situ Detection or Transcription in Transrected Cells Using Biotin·Labeled Molecular Probes......Page 565 RNA Delection and Localization in Cells and Tissue Sections by in Situ Hybridization of 35S-Labeled RNA Probes......Page 574 Electrophoretic Assay for DNA-Binding Proteins......Page 586 Author Index......Page 602