Mitosis and Meiosis 9780080859590, 9780125441636, 0125441630

Table of contents :
Content:
Series Editors
Page ii

Edited by
Page iii

Copyright page
Page iv

Contributors
Pages xi-xiii

Preface
Page xv

Chapter 1 Isolation of Centrosomes from Drosophila Embryos Original Research Article
Pages 1-12
Michelle Moritz, Bruce M. Alberts

Chapter 2 Studying the Composition and Function of Centrosomes in Vertebrates Original Research Article
Pages 13-34
Michel Bornens, Mohammed Moudjou

Chapter 3 Isolation of Centrosomes from Spisula solidissima Oocytes Original Research Article
Pages 35-56
Robert E. Palazzo, Jacalyn M. Vogel

Chapter 4 Methods for in Situ Localization of Proteins and DNA in the Centromere—Kinetochore Complex Original Research Article
Pages 57-80
A. Van Hooser, William R. Brinkley

Chapter 5 Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research Original Research Article
Pages 81-111
Bruce F. McEwen, Michael Marko

Chapter 6 Enlightening Mitosis: Construction and Expression of Green Fluorescent Fusion Proteins Original Research Article
Pages 113-135
Kevin F. Sullivan

Chapter 7 Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes Original Research Article
Pages 137-143
Torsten Wittmann, Tony Hyman

Chapter 8 In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity Original Research Article
Pages 145-153
Fedor Severin, Ken Kaplan, Peter Sorger, Tony Hyman

Chapter 9 Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells Original Research Article
Pages 155-173
Clare Waterman-Storer, Arshad Desai, E.D. Salmon

Chapter 10 Polarized Light Microscopy of Spindles Original Research Article
Pages 175-208
Rudolf Oldenbourg

Chapter 11 Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes Original Research Article
Pages 209-218
Dahong Zhang, R. Bruce Nicklas

Chapter 12 Microinjection of Mitotic Cells Original Research Article
Pages 219-231
Patricia Wadsworth

Chapter 13 Obtaining Antibodies to Spindle Components Original Research Article
Pages 233-244
Ryoko Kuriyama, Kathy Ensrud

Chapter 14 Using Antisense Technology to Study Mitosis Original Research Article
Pages 245-266
Linda Wordeman, Mike Wagenbach

Chapter 15 The Use and Action of Drugs in Analyzing Mitosis Original Research Article
Pages 267-295
Mary Ann Jordan, Leslie Wilson

Chapter 16 Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures Original Research Article
Pages 297-315
Conly L. Rieder, Grisel Cassels

Chapter 17 Identification and Characterization of Mitotic Mutations in Drosophila Original Research Article
Pages 317-346
William E. Theurkauf, Margarete M.S. Heck

Chapter 18 Methods for Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans Original Research Article
Pages 347-368
Berl R. Oakley

Chapter 19 Using Green Fluorescent Protein Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast Original Research Article
Pages 369-383
Kerry S. Bloom, Dale L. Beach, Paul Maddox, Sidney L. Shaw, Elaine Yeh, Edward D. Salmon

Chapter 20 The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in Vitro Original Research Article
Pages 385-412
Arshad Desai, Andrew Murray, Timothy J. Mitchison, Claire E. Walczak

Chapter 21 Methods for Studying Cell Division in Higher Plants Original Research Article
Pages 413-437
Jan W. Vos, Aline H. Valster, Peter K. Hepler

Chapter 22 Using Sea Urchin Gametes for the Study of Mitosis Original Research Article
Pages 439-472
Greenfield Sluder, Frederick J. Miller, Edward H. Hinchcliffe

Index
Pages 473-489