Methods in Plant Cell Biology [1 ed.] 9780080859484, 9780125641524, 0125641524

Table of contents :
Content:
Series Editors
Page ii

Edited by
Page iii

Copyright Page
Page iv

Contributors
Pages xv-xix

Preface
Pages xxi-xxii
David W. Galbraith, Don P. Bourque, Hans J. Bohnert

Chapter 1 Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green-Fluorescent Protein Original Research Article
Pages 3-14
David W. Galbraith, Georgina M. Lambert, Robert J. Grebenok, Jen Sheen

Chapter 2 Functional Effects of Structural Changes in Photosystem II as Measured by Chlorophyll Fluorescence Kinetics Original Research Article
Pages 15-30
Wim F.J. Vermaas

Chapter 3 Determination of Protein Isoprenylation in Vitro and in Vivo Original Research Article
Pages 31-39
Jian-Kang Zhu, Ray A. Bressan, Paul M. Hasegawa

Chapter 4 Extraction and Assay of Protein from Single Plant Cells Original Research Article
Pages 41-49
William H. Outlaw Jr.

Chapter 5 Photoaffinity Labeling and Strategies for Plasma Membrane Protein Purification Original Research Article
Pages 51-60
Joachim Feldwisch, Andreas Vente, Narciso Campos, Rolf Zettl, Klaus Palme

Chapter 6 Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants Original Research Article
Pages 61-83
Sergio Lucretti, Jaroslav Doležel

Chapter 7 Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology Original Research Article
Pages 85-99
William L. Crosby, Peter Schorr

Chapter 8 Isolation and Characterization of Plant Nuclei Original Research Article
Pages 101-112
Tom J. Guilfoyle

Chapter 9 Isolation of Nuclei Suitable for in Vitro Transcriptional Studies Original Research Article
Pages 113-128
John C. Cushman

Chapter 10 Analysis of the H+ATPase and Other Proteins of the Arabidopsis Plasma Membrane Original Research Article
Pages 129-130,130a,130b,131-148
G.Eric Schaller, Natalie D. DeWitt

Chapter 11 Isolation and Functional Reconstitution of the Vacuolar H'-ATPase Original Research Article
Pages 149-160
John M. Ward, Heven Szet

Chapter 12 Isolation and Fractionation of Plant Mitochondria and Chloroplasts: Specific Examples Original Research Article
Pages 161-175
José Manuel Gualberto, Hirokazu Handa, Jean Michel Grienenberger

Chapter 13 Isolating the Plant Mitotic Apparatus: A Procedure for Isolating Spindles from the Diatom set Cylindrotheca fusiformis Original Research Article
Pages 177-187
Harrison Wein, Barbara Brady, W.Zacheus Cande

Chapter 14 Chromoplasts Original Research Article
Pages 189-207
C.A. Price, Noureddine Hadjeb, Lee A. Newman, Ellen M. Reardon

Chapter 15 Methods for Isolation and Analysis of Polyribosomes Original Research Article
Pages 209-222
Eric Davies, Shunnosuke Abe

Chapter 16 Methods for Isolation and Analysis of the Cytoskeleton Original Research Article
Pages 223-236
Shunnosuke Abe, Eric Davies

Chapter 17 Isolation and Characterization of Plasmodesmata Original Research Article
Pages 237-253
Bernard L. Epel, Bella Kuchuck, Guy Kotlizky, Shomrat Shurt, Michael Erlanger, Avital Yahalom

Chapter 18 Characterization and Isolation of the Chloroplast Protein Import Machinery Original Research Article
Pages 255-267
Karin Waegemann, Jürgen Soll

Chapter 19 Macromolecular Movement into Mitochondria Original Research Article
Pages 269-281
Elzbieta Glaser, Carina Knorpp, Marie Hugosson, Erik von Stedingk

Chapter 20 Targeting of Proteins to the Nucleus Original Research Article
Pages 283-294
James C. Carrington

Chapter 21 Import into the Endoplasmic Reticulum Original Research Article
Pages 295-308
Aldo Ceriotti, Emanuela Pedrazzini, Marcella De Silvestris, Alessandro Vitale

Chapter 22 Protein—Protein Interactions within the Endoplasmic Reticulum Original Research Article
Pages 309-323
Jeffrey W. Gillikin, Elizabeth P.B. Fontes, Rebecca S. Boston

Chapter 23 Assaying Proteins for Molecular Chaperone Activity Original Research Article
Pages 325-334
Garrett J. Lee

Chapter 24 The Use of Protoplasts to Study Protein Synthesis and Transport by the Plant Endomembrane System Original Research Article
Pages 335-348
Jürgen Denecke, Alessandro Vitale

Chapter 25 Assessment of Translational Regulation by Run-off Translation of Polysomes In Vitro Original Research Article
Pages 349-359
Michael E. Vayda

Chapter 26 Electroporation of Plant Protoplasts and Tissues Original Research Article
Pages 363-373
George W. Bates

Chapter 27 Particle Bombardment Original Research Article
Pages 375-382
Paul Christou

Chapter 28 Preparation and Transformation of Monocot Protoplasts Original Research Article
Pages 383-399
C. Maas, C. Reichel, J. Schell, H.-H. Steinbiβ

Chapter 29 Tobacco Protoplast Transformation and Use for Functional Analysis of Newly Isolated Genes and Gene Constructs Original Research Article
Pages 401-410
Regina Fischer, Rüdiger Hain

Chapter 30 Novel Inducible/Repressible Gene Expression Systems Original Research Article
Pages 411-424
Christiane Gatz

Chapter 31 Reporter Genes Original Research Article
Pages 425-438
Clemens Suter-Crazzolara, Manfred Klemm, Bernd Reiss

Chapter 32 Cell-Specific Ablation in Plants Original Research Article
Pages 439-448
Mary K. Thorsness, June B. Nasrallah

Chapter 33 Ribozymes Original Research Article
Pages 449-460
Peter Steinecke, Peter H. Schreier

Chapter 34 Expression of Plant Proteins in Baculoviral and Bacterial Systems Original Research Article
Pages 461-479
Reinhard Kunze, Heidi Fuβwinkel, Siegfried Feldmar

Chapter 35 Expression and Localization of Plant Membrane Proteins in Saccharomyces Original Research Article
Pages 481-496
Ramón Serrano, José-Manuel Villalba

Chapter 36 Synthesis of Plant Proteins in Heterologous Systems: Xenopus laevis Oocytes Original Research Article
Pages 497-517
Gad Galili, Yoram Altschuler, Aldo Ceriotti

Chapter 37 Heterologous Expression of Higher Plant Transport Proteins and Repression of Endogenous Ion Currents in Xenopus Oocytes Original Research Article
Pages 519-533
Julian I. Schroeder

Index
Pages 535-549

Volumes In Series
Pages 551-555