Metallobiochemistry Part D: Physical and Spectroscopic Methods for Probing Metal Ion Environment in Metalloproteins 9780121821289, 0121821285
This volume of Methods in Enzymology and its companion Volume 226 present spectroscopic and physical methods for the det
320 58 251KB
English Pages 665 Year 1993
Table of contents :
Preface......Page 1
[1] Two-Dimensional Nuclear Magnetic Resonance of Paramagnetic Metalloproteins......Page 3
[2] Cadmium-113 Nuclear Magnetic ResonanceApplied to Metalloproteins......Page 19
[3] Lanthanide Shift Reagents......Page 47
[4] Alkali Metal Nuclear Magnetic Resonance......Page 83
[5] Calcium Nuclear Magnetic Resonance......Page 112
[6] Pulsed Electron Nuclear Multiple ResonanceSpectroscopic Methods for Metalloproteinsand Metalloenzymes......Page 124
[7] Continuous Wave Electron Nuclear Double Resonance Spectroscopy......Page 196
[8] Vanadyl(IV) Electron Nuclear Double Resonance/Electron Spin Echo Envelope Modulation Spin Probes......Page 238
[9] Multidimensional Nuclear Magnetic Resonance Methodsto Probe Metal Environments in Proteins......Page 251
[10] Optically Detected Magnetic Resonance of Triplet States in Proteins......Page 298
[11] Electron Paramagnetic Resonance......Page 339
[12] Electron Paramagnetic Resonance Spectroscopyof Iron Complexes and Iron-Containing Proteins......Page 363
[13] Intrinsic and Extrinsic Paramagnets as Probes of Metal Clusters......Page 395
[14] Electron Paramagnetic Resonance Spectroelectrochemical Titration......Page 407
[15] Magnetic Susceptibility......Page 423
[16] Multifield Saturation Magnetization of Metalloproteins......Page 449
[17] Combining M6ssbauer Spectroscopy with Integer SpinElectron Paramagnetic Resonance......Page 476
[18] Voltammetric Studies of Redox-Active Centersin Metalloproteins Adsorbed on Electrodes......Page 493
[19] Direct and Indirect Electrochemical Investigations of MetaUoenzymes......Page 515
[20] Pulse Radiolysis......Page 537
[21] Physical Methods to Locate Metal Atoms in Biological Systems......Page 550
Contributors to Volume 227......Page 602
Author Index......Page 618
Subject Index......Page 643
Preface......Page 1
[1] Two-Dimensional Nuclear Magnetic Resonance of Paramagnetic Metalloproteins......Page 3
[2] Cadmium-113 Nuclear Magnetic ResonanceApplied to Metalloproteins......Page 19
[3] Lanthanide Shift Reagents......Page 47
[4] Alkali Metal Nuclear Magnetic Resonance......Page 83
[5] Calcium Nuclear Magnetic Resonance......Page 112
[6] Pulsed Electron Nuclear Multiple ResonanceSpectroscopic Methods for Metalloproteinsand Metalloenzymes......Page 124
[7] Continuous Wave Electron Nuclear Double Resonance Spectroscopy......Page 196
[8] Vanadyl(IV) Electron Nuclear Double Resonance/Electron Spin Echo Envelope Modulation Spin Probes......Page 238
[9] Multidimensional Nuclear Magnetic Resonance Methodsto Probe Metal Environments in Proteins......Page 251
[10] Optically Detected Magnetic Resonance of Triplet States in Proteins......Page 298
[11] Electron Paramagnetic Resonance......Page 339
[12] Electron Paramagnetic Resonance Spectroscopyof Iron Complexes and Iron-Containing Proteins......Page 363
[13] Intrinsic and Extrinsic Paramagnets as Probes of Metal Clusters......Page 395
[14] Electron Paramagnetic Resonance Spectroelectrochemical Titration......Page 407
[15] Magnetic Susceptibility......Page 423
[16] Multifield Saturation Magnetization of Metalloproteins......Page 449
[17] Combining M6ssbauer Spectroscopy with Integer SpinElectron Paramagnetic Resonance......Page 476
[18] Voltammetric Studies of Redox-Active Centersin Metalloproteins Adsorbed on Electrodes......Page 493
[19] Direct and Indirect Electrochemical Investigations of MetaUoenzymes......Page 515
[20] Pulse Radiolysis......Page 537
[21] Physical Methods to Locate Metal Atoms in Biological Systems......Page 550
Contributors to Volume 227......Page 602
Author Index......Page 618
Subject Index......Page 643
- Author / Uploaded
- John N. Abelson (Editor)
- James F. Riordan (Editor)
- Bert L. Vallee (Editor)
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