Isolated Hepatocytes Preparation, Properties and Applications [1 ed.] 9780080858906, 9780444813022, 0444813020
This book sets out clearly and effectively the preparation and working methods of laboratory techniques involving isolat
271 74 273KB
English Pages ii-xxi, 1-460 [483] Year 1991
Table of contents :
Content:
Edited By
Page ii
Copyright page
Page iv
Preface
Pages v-vii
Michael N. Berry
Acknowledgements
Page ix
Abbreviations
Page xxi
Chapter 1 Background
Pages 1-14
Chapter 2 High-yield preparation of isolated hepatocytes from rat liver
Pages 15-58
Chapter 3 Other methods for hepatocyte isolation
Pages 59-81
Chapter 4 Assessment of integrity of isolated hepatocytes
Pages 83-98
Chapter 5 Microscopy of isolated hepatocytes
Pages 99-120
Chapter 6 Biochemical properties
Pages 121-178
Chapter 7 Utilization of hepatocytes for drug studies
Pages 179-200
Chapter 8 Study of the effects of hormones
Pages 201-214
Chapter 9 Ca2+ ion transport and compartmentation
Pages 215-236
Chapter 10 Hepatocyte isolation for primary culture and methods for non-adherent culture
Pages 237-263
Chapter 11 Monolayer culture of hepatocytes
Pages 265-354
Chapter 12 Selected specialized techniques
Pages 355-400
Appendix 1
Pages 401-402
Appendix 2
Pages 403-407
References
Pages 409-439
Subject index
Pages 441-460
Content:
Edited By
Page ii
Copyright page
Page iv
Preface
Pages v-vii
Michael N. Berry
Acknowledgements
Page ix
Abbreviations
Page xxi
Chapter 1 Background
Pages 1-14
Chapter 2 High-yield preparation of isolated hepatocytes from rat liver
Pages 15-58
Chapter 3 Other methods for hepatocyte isolation
Pages 59-81
Chapter 4 Assessment of integrity of isolated hepatocytes
Pages 83-98
Chapter 5 Microscopy of isolated hepatocytes
Pages 99-120
Chapter 6 Biochemical properties
Pages 121-178
Chapter 7 Utilization of hepatocytes for drug studies
Pages 179-200
Chapter 8 Study of the effects of hormones
Pages 201-214
Chapter 9 Ca2+ ion transport and compartmentation
Pages 215-236
Chapter 10 Hepatocyte isolation for primary culture and methods for non-adherent culture
Pages 237-263
Chapter 11 Monolayer culture of hepatocytes
Pages 265-354
Chapter 12 Selected specialized techniques
Pages 355-400
Appendix 1
Pages 401-402
Appendix 2
Pages 403-407
References
Pages 409-439
Subject index
Pages 441-460
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