Density Gradient Centrifugation 9780720442212, 0720442214
232 89 305KB
English Pages ii-vi, 1-534 [543] Year 1978
Table of contents :
Content:
Edited by
Page ii
Copyright Page
Page iv
Editors' preface
Pages v-vi
Density Gradient Centrifugation Original Research Article
Page 1
Richard Hinton, Miloslav Dobrota
Chapter 1 Introduction to zonal centrifugation
Pages 8-45
Chapter 2 Theoretical aspects of centrifugal separations
Pages 46-69
Chapter 3 Conditions for a centrifugal separation
Pages 70-96
Chapter 4 Centrifugation in conventional rotors
Pages 97-119
Chapter 5 Centrifugation in zonal rotors
Pages 120-196
Chapter 6 Assay of fractions separated by density gradient centrifugation
Pages 197-204
Chapter 7 Applications of density gradient centrifugation
Pages 205-242
Chapter 8 Artefacts arising during centrifugal separations
Pages 243-253
Chapter 9 Future prospects for density gradient centrifugation
Pages 254-261
Acknowledgements
Page 262
Appendices
Pages 263-273
Refrences
Pages 274-286
Subject Index
Pages 287-290
An Introduction To Radioimmunoassay and Related Techniques Original Research Article
Page 291
T. Chard
List of abbreviations
Pages 299-300
Chapter 1 The background to radioimmunoassay
Pages 301-328
Chapter 2 Requirements for a binding assay - purified ligand
Pages 329-342
Chapter 3 Requirements for binding assays - tracer ligand
Pages 343-376
Chapter 4 Requirements for a binding assay - the binder
Pages 377-400
Chapter 5 Requirements for a binding assay - separation of bound and free ligand
Pages 401-426
Chapter 6 Requirements for a binding assay - extraction of ligand from biological fluids
Pages 427-439
Chapter 7 Requirements for binding assays - calculation of results
Pages 440-445
Chapter 8 Characteristics of binding assays - sensitivity
Pages 446-462
Chapter 9 Characteristics of binding assays - specificity
Pages 463-478
Chapter 10 Characteristics of binding assays - precision
Pages 479-494
Chapter 11 Characteristics of binding assays - relation to other types of assay
Pages 495-503
Chapter 12 Automation of binding assays
Pages 504-509
Chapter 13 Organisation of assay services
Pages 510-517
Appendices
Pages 518-526
References
Pages 527-530
Subject index
Pages 531-534
Content:
Edited by
Page ii
Copyright Page
Page iv
Editors' preface
Pages v-vi
Density Gradient Centrifugation Original Research Article
Page 1
Richard Hinton, Miloslav Dobrota
Chapter 1 Introduction to zonal centrifugation
Pages 8-45
Chapter 2 Theoretical aspects of centrifugal separations
Pages 46-69
Chapter 3 Conditions for a centrifugal separation
Pages 70-96
Chapter 4 Centrifugation in conventional rotors
Pages 97-119
Chapter 5 Centrifugation in zonal rotors
Pages 120-196
Chapter 6 Assay of fractions separated by density gradient centrifugation
Pages 197-204
Chapter 7 Applications of density gradient centrifugation
Pages 205-242
Chapter 8 Artefacts arising during centrifugal separations
Pages 243-253
Chapter 9 Future prospects for density gradient centrifugation
Pages 254-261
Acknowledgements
Page 262
Appendices
Pages 263-273
Refrences
Pages 274-286
Subject Index
Pages 287-290
An Introduction To Radioimmunoassay and Related Techniques Original Research Article
Page 291
T. Chard
List of abbreviations
Pages 299-300
Chapter 1 The background to radioimmunoassay
Pages 301-328
Chapter 2 Requirements for a binding assay - purified ligand
Pages 329-342
Chapter 3 Requirements for binding assays - tracer ligand
Pages 343-376
Chapter 4 Requirements for a binding assay - the binder
Pages 377-400
Chapter 5 Requirements for a binding assay - separation of bound and free ligand
Pages 401-426
Chapter 6 Requirements for a binding assay - extraction of ligand from biological fluids
Pages 427-439
Chapter 7 Requirements for binding assays - calculation of results
Pages 440-445
Chapter 8 Characteristics of binding assays - sensitivity
Pages 446-462
Chapter 9 Characteristics of binding assays - specificity
Pages 463-478
Chapter 10 Characteristics of binding assays - precision
Pages 479-494
Chapter 11 Characteristics of binding assays - relation to other types of assay
Pages 495-503
Chapter 12 Automation of binding assays
Pages 504-509
Chapter 13 Organisation of assay services
Pages 510-517
Appendices
Pages 518-526
References
Pages 527-530
Subject index
Pages 531-534
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