Analytical and Preparative Separation Methods of Biomacromolecules [1 ed.] 0824719964, 9780824719968
Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides informati
207 20 287KB
English Pages 476 Year 1999
Table of contents :
Preface......Page 6
Contents......Page 8
Contributors......Page 12
Analysis of HbA1c and Some Hb Variants by H PLC......Page 15
Analysis of Posttranslational Modifications in Recombinant Proteins by HPLC and Mass Spectrometry......Page 27
Analytical and Preparative Separations of Peptides by Capillary and Free-Flow Zone Electrophoresis......Page 53
Bioaffinity Chromatography......Page 113
Characterization and Partial Purification of Steroidogenic Factors from Thymic Epithelial Cell-Conditioned Medium......Page 181
Determination of Affinity Constants of Lectins for Sugars by Affinity Capillary Electrophoresis......Page 201
Displacement Chromatography of Biomolecules......Page 217
High-Performance Membrane Chromatography of Proteins......Page 269
HPLC Purification of Recombinant Proteins......Page 315
Isolation, Purification, and Characterization of Human Seminal Plasma Proteins and Their immunological Behavior In Vitro......Page 345
Isolation, Purification, and Structural Study of Allergenic Proteins......Page 367
Multiangle Combined Light Scattering with HPLC with Examples for Biopolymers......Page 383
Purification and Characterization of Connective Tissue Growth Factor Using Heparin Affinity Chromatography......Page 411
Slalom Chromatography......Page 429
Simultaneous Chromatographic Separation of Ceruloplasmin and Serum Amine Oxidase......Page 445
Fast, Single-Step Affinity Chromatography Purification of Hemoglobin......Page 459
Index......Page 471
Preface......Page 6
Contents......Page 8
Contributors......Page 12
Analysis of HbA1c and Some Hb Variants by H PLC......Page 15
Analysis of Posttranslational Modifications in Recombinant Proteins by HPLC and Mass Spectrometry......Page 27
Analytical and Preparative Separations of Peptides by Capillary and Free-Flow Zone Electrophoresis......Page 53
Bioaffinity Chromatography......Page 113
Characterization and Partial Purification of Steroidogenic Factors from Thymic Epithelial Cell-Conditioned Medium......Page 181
Determination of Affinity Constants of Lectins for Sugars by Affinity Capillary Electrophoresis......Page 201
Displacement Chromatography of Biomolecules......Page 217
High-Performance Membrane Chromatography of Proteins......Page 269
HPLC Purification of Recombinant Proteins......Page 315
Isolation, Purification, and Characterization of Human Seminal Plasma Proteins and Their immunological Behavior In Vitro......Page 345
Isolation, Purification, and Structural Study of Allergenic Proteins......Page 367
Multiangle Combined Light Scattering with HPLC with Examples for Biopolymers......Page 383
Purification and Characterization of Connective Tissue Growth Factor Using Heparin Affinity Chromatography......Page 411
Slalom Chromatography......Page 429
Simultaneous Chromatographic Separation of Ceruloplasmin and Serum Amine Oxidase......Page 445
Fast, Single-Step Affinity Chromatography Purification of Hemoglobin......Page 459
Index......Page 471
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